Bio Hacking Health

How to Mix & Reconstitute Peptides Safely (With Step-by-Step Guide)

Why Correct Reconstitution Matters

Peptides are delicate and must be reconstituted properly to maintain potency. Incorrect mixing can lead to:

  • reduced effectiveness
  • wasted product
  • bacterial contamination
  • inaccurate dosing

This guide ensures safe, accurate preparation every time.


You’ll Need:

  • Peptide vial (Semaglutide / Tirzepatide / Retatrutide)
  • Bacteriostatic water
  • Alcohol swabs
  • Sterile insulin syringes (1mL)

Step-by-Step Mixing Instructions

Step 1 — Clean Your Workspace

Wipe your area with alcohol to prevent contamination.

Step 2 — Remove Caps & Sanitize

Clean both vial tops with an alcohol swab.

Step 3 — Draw Bacteriostatic Water

Use a sterile syringe to pull the recommended amount (usually 1–2mL depending on concentration).

Step 4 — Inject Into Peptide Vial Slowly

Let the water slide down the inside wall of the vial—never shake.

Step 5 — Swirl Gently

Rotate the vial until fully dissolved.

Step 6 — Store Properly

Refrigerate the vial. Use within 30–60 days for best potency.

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